Richard Allen (University of Hawaii) (2011) CIL:1307, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

High resolution image of immunogold labeled microtubules next to the cytoproct radiate from basal bodies and also from the tip of the cytoproct. These microtubules lie along the approaching spent DV. b-tubulin immunogold labeled TEM image taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with a Nikon Coolscan 9000ED. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

高分辨率免疫金标记的微管图像，位于细胞质突的辐射区域，这些区域既源自基体，也源自细胞质突的尖端。这些微管沿着即将耗尽的DV接近。图像为R. Allen于1996年7月5日使用Zeiss 10A在80kV下拍摄的b-微管免疫金标记透射电镜（TEM）图像。负片放大倍数为12,000倍。细胞经过0.25%戊二醛轻微固定，并用2.3M蔗糖渗透，随后在液氮中冷冻，并在-100°C的温度下切成约75nm厚的薄片。这些制备的冷冻切片随后被解冻、清洗，并暴露于由小鼠或兔/山羊制备的单克隆一级抗体，以及胶体金复合的山羊抗小鼠/兔二级抗体。制备的详细情况见《细胞生物学方法》2010年第96卷第143-173页。原始胶片使用Nikon Coolscan 9000ED扫描。此图像最适宜进行定量分析。更多信息请参考（http://www5.pbrc.hawaii.edu/allen/）。