Single-cell Atlas Reveals Fatty Acid Metabolites Regulate the Functional Heterogeneity of Mesenchymal Stem Cells

Here, using scRNA-seq on bone marrow MSCs, we identified pre-MSCs and lineage-committed MSC clusters, and revealed their potential regulatory mechanisms in MSC lineage commitment. Our data indicated a novel potential role of MSCs responding to fatty acid treatment, which could be applied to in vitro culture. Single-cell RNA-seq on bone marrow MSCs本研究通过对骨髓间充质干细胞（bone marrow MSCs）开展单细胞RNA测序（single-cell RNA-seq, scRNA-seq），鉴定得到前体间充质干细胞（pre-MSCs）与谱系定型间充质干细胞簇，并阐明了其在间充质干细胞谱系定型过程中的潜在调控机制。本研究数据表明，间充质干细胞在响应脂肪酸处理时存在新型潜在作用，该发现可应用于体外培养体系。骨髓间充质干细胞单细胞RNA测序