A Conjugated Polymer-Based Injectable Hydrogel for Programmed Antibiofilm and Anti-Inflammation Therapy to Promote Diabetic Wound Healing

Chronic diabetic wounds pose severe
challenges in the
clinic due
to drug-resistant biofilm infections and an inflammatory microenvironment.
Herein, an injectable hydrogel (NPs@gel) is developed and applied
for antibiofilm and anti-inflammation, accelerating diabetic wound
healing. First, an o-nitrobenzene (NB)-modified conjugated polyfluorene
vinylene photosensitizer (PFV-NB) is designed and synthesized. Then,
PFV-CA NPs are prepared, containing PFV-NB, chloramphenicol, and astaxanthin
(AST), followed by the construction of NPs@gel. Notably, under light
irradiation, PFV-NB can generate aldehyde groups that adhere to the
biofilm through the Schiff base reaction, inhibiting the bacterial
drug efflux pump, resensitizing the chloramphenicol, and damaging
the biofilm. Meanwhile, PFV-NB can produce reactive oxygen species,
further enabling photodynamic therapy and antibiofilm activity. Also,
AST can alleviate inflammation after antibacterial treatment and regulate
the diabetic wound microenvironment. Finally, NPs@gel can perfectly
cover irregular wounds and realize programmed diabetic wound therapy.
This work provides a promising synergistic strategy and material for
the efficient treatment of chronic wounds.