Combining CDK12 and BAF inhibition synergistically impairs cancer cell survival in triple-negative breast cancer line

Chromatin profiling with CUT&Tag and CUTAC reveals that while CDK12 inhibition primarily disrupts DNA accessibility at Brg1 and CDK12 co-occupied promoters, BAF inhibition leads to widespread loss of enhancer accessibility. Co-inhibition causes a compounded disruption at shared co-occupied regulatory sites, resulting in loss of chromatin accessibility and transcriptional dysregulation at key loci. Overall design: CUT&Tag uses tagmentation in which the barcoded adapters are integrated on both sides of the insert, so that the DNA that is released is already a barcoded library.