台灣松露之栽培與產業化研究(3/3)The Cultivation and Industrialization of Truffles in Taiwan (3/3)

進行一年生棵青剛櫟半無菌苗之接種前檢定工作。確認這些半無菌苗根部未受其他的真菌感染。將培育完成的青剛櫟半無菌苗進行松露孢子懸浮液的接種工作。嘗試並評估各種接種方式，以找出最方便且高成功率的接種方式。接種試驗濃度：分別以1000、2000、4000及8000孢子/毫升4種不同孢子懸浮液濃度進行接種試驗。接種方式：有兩種，分別為（一）以穴植盤浸泡各濃度之孢子懸浮液5分鐘的接種方式進行接種。（二）單株個別接種方式，接種各種孢子懸浮液各1毫升。目前評估結果，以穴植盤浸泡各濃度之孢子懸浮液的接種方式效果不佳，幾乎沒有發現感染的植株，平均感染比例為1.25％。以單株個別接種方式接種各種孢子懸浮液各1毫升，以8000個/毫升的接種成功比率最高，其次為4000，再其次為2000及1000；成功比率分別為22％、17％，12％及8％。完成接種的苗木則進行施肥撫育，分別以施用100天肥效之好康多、每兩星期施用花寶2號及對照組進行生長評估。發現施用肥效100天的好康多對苗木的生長效果最好。水源地苗圃內的青剛櫟菌根苗遭受小白紋毒蛾的取食，以施用好康多的苗木受害最嚴重。其次是施用花寶的菌根苗。作為對照組的菌根苗，雖然生長比不上其他施肥的菌根苗，但是明顯受害最為輕微。Verification work was conducted by check the roots of these one-year-old semi-steriled Quercus glauca seedlings were colonization by other mycorrizal fungi before the inoculation test. These semi-steriled seedlings were inocualted by spore suspension of Tuber indiucm. Two inoculated methods were conducted. (1) polypaks with seedlings was soaked with different concentrations of spore suspension for 5min ；(2)Individual plant was inoculated with 1ml spore supension of a variety of spore suspension. Assessment results to show the mehod of polypake-soaking is ineffective, almost did not find mycorrizal plants, the average infection ratio was 1.25％. Adjusted spore suspension into 8000，4000, 2000 and 1000 spores/ml, and inoculated 1ml spore suspension on the roots of individual plant. 8000 spores/ ml inoculum has the highest success rate, followed by 4000, 2000 and 1000 and then followed; success rates were 22％, 17％, 12％ and 8％. Mycorrizal plants were fertilized with 3 ways, one is fertilized by 100-day long-acting fertilizer , one is spraying diluted chemical fertilizer very 2 weeks, and one is no top dressing as control. Resurlt of top dressing test showed 100-days long-acting fertilizer show the best effect on the growth of seedlings. and then fellowed by spraying diluted chemical fertilizer very 2 weeks and control. The better growed mycorrhizal Quercus glauca seedling,suffered more serious by Small tussock moth in the nursey.