Effect of IFNa2 treatment in affecting gene expression in monocytes and the differential gene expression between the monocytes from healthy donors and SLE patients

To investigate the effect of IFNa2 treatment in CD14+ monocytes, monocytes isolated from healthy donors were treated with or without IFNa2, and SLE monocytes were also harvested for the comparison of the differential expression genes. Overall design: We performed gene expression profiling analysis using data obtained from RNA-seq of three groups including healthy monocyte, IFNA2-treated monocytes (for 6 hours) and SLE monocytes. Comparative gene expression profiling analysis of RNA-seq data for human CD14+ monocyte and the IFN-a2 treated monocyte and CD14+ monocyte from SLE patients.