H3K27ac and p300 cut&run experiment in G549 (Injury-response/Mesenchymal GSC) cell line

EP300 was identified as potential regulator of Injury-response/Mesenchymal state in Glioblastoma stem cells. Our objective was to evaluate the effect of EP300_KO on H3K27Ac within the genome in Injury-response GSC. Moreover, we aimed to identify the p300 binding sites in Injury-response GSC to further assess how it regulates the Injury-response state. Overall design: CUT&RUN experiment for histone modification H3K27ac in AAVS1 and EP300 KO G549 Cells CUT&RUN experiment for p300 binding sites in WT G549 cells