rCRUX Generated MiSebastes Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: MiSebastes<br> Gene: CytB<br> Length of Target: 153<br> get_seeds_local() minimum length: 107<br> get_seeds_local() maximum length: 199<br> blast_seeds() minimum length: 71<br> blast_seeds() maximum length: 163<br> max_to_blast: 100<br> Forward Sequence (5'-3'): AAGCTCATTCAAGTGCTT<br> Reverse Sequence (5'-3'): GACCACTTACACAATTCT<br> Reference: Min, M. A., Barber, P. H., &amp; Gold, Z. (2021). MiSebastes: An eDNA metabarcoding primer set for rockfishes (genus Sebastes). Conservation Genetics Resources, 13(4), 447-456. https://doi.org/10.1007/s12686-021-01219-2 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.