Analysis of proteins domains of Src involved in Pyk2 phosphorylation.
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https://figshare.com/articles/dataset/_Analysis_of_proteins_domains_of_Src_involved_in_Pyk2_phosphorylation_/1645142
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A, SYF cells transiently transfected with Src-WT or the various Src mutant constructs were serum-starved, detached and kept in suspension in DMEM containing 0.5% BSA for 1 hr. Cells were then seeded on fibronectin-coated dishes, and allowed to attach for 30 min. Phosphorylation of Pyk2 and FAK was examined by immunoblot. B, Co-IP of Src and Pyk2. SYF cells were transiently transfected with Myc-Pyk2 along with Src-WT or Src mutant constructs, the expressed Src was immunoprecipitated and the associated Pyk2 was examined by immunoblot.
创建时间：
2016-03-16
