Bcl11a is essential for B-1a cell maintenance during aging (RNA-Seq)

B-1a cells are important immune cells, serving as the first line of defense against pathogens. B-1a cell undergo a series of alterations during aging and decrease the protection to our body. However, the characteristics of B-1a cells during the aging process are not fully understood. In this study, we describe transcriptional and epigenetic profiles of B-1a from 3-month-old and 24-month-old mice across both genders. Interestingly we found that the expression of the transcription factor Bcl11a was positively correlated with the number of B-1a cells in aged male and aged female mice.To further characterize senescent B-1a cells and the mechanisms of Bcl11a regulates B-1a. ATAC-seq and RNA-seq were performed on the B-1a isolated from young male mice (3-month-old),old male mice (24-month-old mice), young female mice (3-month-old),old female mice (24-month-old mice) of C57BL/6,and Bcl11a delated young male mice. CUT&tag was performed on the B-1a isolated from young male mice. Collectively, we provide a comprehensive resource to decode the aging process of B-1a, shedding light on how Bcl11a regulate B-1a cells maintenance. RNA-seq were performed on the B-1a isolated from young male mice (3-month-old),old male mice (24-month-old mice), young female mice (3-month-old),old female mice (24-month-old mice) of C57BL/6,and Bcl11a deleted young male mice.B-1a细胞（B-1a cells）是一类重要的免疫细胞，作为抵御病原体的第一道防线发挥功能。B-1a细胞在衰老进程中会发生一系列改变，其对机体的保护作用逐渐减弱。然而，目前学界对B-1a细胞在衰老过程中的特征尚未完全明晰。本研究针对不同性别、3月龄与24月龄小鼠体内的B-1a细胞，分析了其转录组与表观基因组谱学特征。值得注意的是，我们发现转录因子Bcl11a的表达水平与老年雄性、雌性小鼠体内的B-1a细胞数量呈正相关。为进一步解析衰老状态下B-1a细胞的特征，以及Bcl11a调控B-1a细胞的分子机制，我们对C57BL/6背景的年轻雄性（3月龄）、年老雄性（24月龄）、年轻雌性（3月龄）、年老雌性（24月龄）小鼠，以及Bcl11a敲除的年轻雄性小鼠体内分离得到的B-1a细胞，进行了ATAC测序（ATAC-seq）与RNA测序（RNA-seq）。同时，我们对年轻雄性小鼠体内分离得到的B-1a细胞开展了CUT&Tag测序（CUT&Tag）。综上，本研究提供了一套可用于解析B-1a细胞衰老进程的综合性组学资源，为阐明Bcl11a调控B-1a细胞稳态维持的机制提供了新的研究视角。我们再次对上述C57BL/6背景的四组小鼠及Bcl11a敲除年轻雄性小鼠体内分离得到的B-1a细胞进行了RNA测序。