Virus detection in tissues from pigs infected with Zaire ebolavirus (ZEBOV).

Virus detection for pigs infected with Zaire ebolavirus in experiment 2 was done by quantitative real time RT-PCR targeting the L gene of ZEBOV and L gene copy numbers estimated based on a standard curve. Data represents log10 copies/mL of tissue suspension (numbers in brackets represent standard deviations of 3 replicates for each sample). SLN, submandibular lymph node; BLN, bronchial lymph node; MLN, mesenteric lymph nodes, U, virus was either absent or below levels detectable by the current assay.