Systematic Dissection of Sequence Elements Controlling s70 Promoters Using a Genomically-Encoded Multiplexed Reporter Assay in E. coli
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资源简介：
We built and tested a library of 10,898 s70 promoter variants consisting of all combinations of a set of eight -35 elements, eight -10 elements, three UP elements, eight spacers, and eight backgrounds. Using a Massively Parallel Reporter Assay, we measure expression of all promoters and evaluate how individual promoter elements contribute to expression. Overall design: We measure expression of all promoters and evaluate how individual promoter elements contribute to expression.
创建时间：
2018-07-02
