Contact angle and surface free energy components of <i>P. aeruginosa</i> PA14 before and after DNase I Treatment and addition of Ca²⁺.

Measurement of contact angles on P. aeruginosa PA14 lawns with water, diiodo-methane and formamide and subsequent surface free energy components: Lifshitz-Van der Waals component (γLW), electron-donor (γ_) and acceptor (γ+) for the acid-base component (γAB) are derived from measured contact angle in the presence and absence of eDNA and Ca2+ (1000 μM). ± represents standard deviations from the mean (n = 4). Data in bold indicate statistically significant (p<0.05) differences between presence and absence of eDNA.