Summary of Trp fluorescence lifetime and anisotropy values of WT and C0 refolded in various LPRs.

aChanges observed in Trp lifetime and anisotropy of the denatured samples can be explained by taking into account the change in viscosity of the sample; b Average tryptophan lifetimes obtained from fits to a three exponential function. The values provided are in ns; c Goodness of fit for the fluorescence lifetime measurements; d Samples have trace amounts of GdnHCl due to direct (D) refolding; 5 mM D2 corresponds to 2 μM protein directly refolded in 5 mM LDAO; e Samples have been diluted from the refolded stock (RF) solution; f ND – Not determined, due to scattering by aggregated protein.