Active soil nitrifying communities revealed by in situ transcriptomics and stable isotope probing
收藏NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA628867
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In the present study, we collected soil samples from 20 field plots under five different fertilization regimes for 22 years. In situ activity of nitrifying communities were assessed high-throughput sequencing of 16S rRNA gene transcript in fresh soils, and DNA-based stable isotope probing (DNA-SIP) was employed to reveal the taxonomic identities of active nitrifiers in microcosms amended with high-concentration urea. Meanwhile, the abundance and composition of nitrifying communities were also assessed in air-dried soils in comparison with RNA and DNA-SIP strategies.
创建时间:
2020-04-28



