Gene regulation by restoration of KMT2C in human colorectal cancer cells HCT116 and RKO

The histone 3 lysine 4 (H3K4) monomethylase KMT2C is mutated across several cancer types, however the effects of mutations on epigenome organization, gene expression and cell growth are not clear. To study effects of KMT2C expression in CRC cells we restored one allele to wild type KMT2C in the two CRC cell lines RKO and HCT116, which both are homozygous KMT2C c.8390delA mutant, by gene editing. RNA sequencing was performed to profile gene expression when KMT2C was restored in RKO and HCT116 cells. Overall design: Three independently isolated RNA samples each were sequenced from parental cell lines HCT116 and RKO. Single samples were sequenced for KMT2C knock-in clones, except RKO KMT2C KI2 where duplicate samples were sequenced. Gene expression levels in KMT2C knock-in clones were compared to those in the respective parental cells.