RNA-seq and ChIP-seq in HepG2 hepatocellular carcinoma and U87 glioblastoma cells with CRISPRi knockdown of ACTR5

This dataset examined the effect of ACTR5 knockdown in HepG2 and U87 cells. The gene expression profiling (RNA-seq) and the chromatin targeting of ACTR5 (TST-ChIP-seq) are reported. RNAseq:dCas9-Krab-expressing HepG2 and U87 cells were transduced with sgiNTC and sgiACTR5. The total RNA was extracted using the RNeasy Mini Kit (74104, QIAGEN) and submitted for RNA-seq at Novogene (PE150, NovoSeq6000). TST-ChIP-seq: ACTR5-TST cautured genomic DNA was submitted for ChIP-seq at Novogene (PE150, NovaSeq6000).