<b>Supplementary data for the small RNA expression analysis in </b><b><i>Gossypium hirsutum</i></b><b> empurpled mutant </b><b><i>HS2</i></b>

Below is a concise overview of the documents that have been released to complement our publication detailing the study on the expression patterns and regulatory functions of small RNAs in the empurpled mutant of Gossypium hirsutum. 1. Assembly.fa The re-constructed transcripts derived from the RNA-seq data of C312 and HS2. These transcripts are used in de novo prediction of miRNAs. 2. Novel miRNAs.docx The pre-sequences and secondary structures of novel miRNAs predicted in our study. 3. Sequences and expression profiles of siRNAs.xls Sequences of all the siRNAs predicted in our study, as well as their expression levels in each library. The expression levels are calculated using reads per million (RPM). 4. DEG_siRNA.xls All the 761 siRNAs that are differentially expressed between C312 and HS2. 5. Regulations.xlsx All the sRNA-target regulations predicted in our study. Only differentially expressed miRNAs and siRNAs were assessed. The mRNAs generated during genome annotation (NAU-NBI_v1.1) are used for target prediction. 6. DEG_mRNA.xlsx The expression patterns of 892 differentially expressed coding genes between C312 and HS2. 7. All expressed miRNAs.xlsx All the miRNAs detected in C312 and HS2 by homology search and de novo prediction. Their expression levels are estimated using RPM.