Data.docx

In
this work, we first use glucose and glycerol as co-carbon source to improve 2′-FL
production from 2.67 to 3.62 g/L. Then, the effect of the deletion of the genes
arcA or iclR on 2′-FL production by E. coli FL-B21 was investigated.
Knockout of arcA and iclR was surprisingly useful for avoiding acetate formation.
Moreover, the arcA mutant FL-011
displayed higher 2′-FL production and higher lactose and glycerol utilization
efficiency than the control strain FL-B21. The 2′-FL production of the arcA mutant FL-011 was 5.04 g/L. To
maximize 2′-FL production by FL-011, systematic optimization of the culture
medium composition and induction conditions was performed. As a result, the
production of 2′-FL reached 7.1 g/L in shake-flask culture.