Dissecting PADI6 function defines oocyte cytoplasmic lattices as regulatory hubs for fundamental cellular processes. Dissecting PADI6 function defines oocyte cytoplasmic lattices as regulatory hubs for fundamental cellular processes

Transcriptome profiling of MII oocytes, zygote and 2-cell embryos (processed as individual blastomeres) from wild-type females, females with a single point mutation in Padi6 resulting in the amino acid substitution C663A (Padi6-C663A), and females with a Padi6 null mutation (Padi6-KO). Overall design: A total of 6 replicates, three from two different litters were processed for each genotype and stage, resulting in a total of six MII oocytes and six zygotes per genotype. The 2-cell embryos were dissociated into individual blastomeres and processed separately resulting in 12 single 2-cell blastomeres per genotype. Samples are numbered by the litter and given a letter indicating the sample from that litter, e.g. C663A_Zygote_1b = Zygote number 2 from the first C663A Zygote litter. 2-cell blastomeres are further differentiated by _b1 or _b2 indicating which blastomere, e.g. WT_2C_1a_b2 = the second blastomere from the first 2-cell embryo from the first wild-type 2-cell embryo litter.