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H3K27Ac ChIP-seq for TL-om1 cells and primary ATL cell samples

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE85692
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ChIP-seq analysis was performed in adult T-cell leukemia/lymphoma cells to analyze acetylation of histone H3K27. TL-Om1 and primary ATL cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
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2019-05-15
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