Gene expression in SAS cell lines upon SNAI1 silencing. Homo sapiens

Collective cell migration is one of the principal modes for cancer cell movements. However, the triggering event for collective migration and its clinical significance is unclear. Here, we found that Snail, a major inducer of epithelial-mesenchymal transition (EMT), is critical for orchestrating collective migration in squamous cell carcinoma (SCC). To invstigate how Snail contribute to collective migration and invasion, we used microarrays to identify the global gene alterations regulated by Snail in SCC cells. Overall design: Control vs. Snail-depleted SAS cells were cultured on thick collagen (2.5D cultured system) for simulating virtual physiological environment. After 24 h, total RNA was harvested for microarray analysis.