Analysis of local mucosal immune dysregulation to guide pilot directed therapy for long-COVID olfactory loss [TCR-seq]

To further define local mechanisms impacting peripheral olfactory structures, we obtained olfactory biopsies from subjects with post-COVID olfactory loss (hyposmia) or controls for viral, single cell and T cell receptor assays. Here we identified clonally expanded T cell subsets with a pro-inflammatory profile. Overall design: Biopsies of human olfactory epithelium collected from three long-COVID hyposmics and three normosmic control patients and processed for 5' GEX and TCR sequencing