Spatial transcriptomic reveals the cellular landscape of bladder cancer

The cellular diversity of stromal compartment within the tumor microenvironment might change dynamically as tumors evolve that allowing distant dissemination of tumor cells and informing the treatment options. Importantly, the localization of cells in tumor microenvironment indicates the biological function of cells. Thus, we used spatial transcriptomic to reveal the cellular landscape and corresponding cellular localization in bladder cancer. The xx-case paraffin sections of bladder cancer were obtained from Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University. Then, the tissue sections were subjected to the Visium Spatial Tissue Optimization Slide kit to fit the time for permeabilization by generating fluorescently labeled cDNA tissue prints. Subsequently, the permeabilization processes were conducted and the spatially barcoded, full-length cDNA is amplified via PCR to generate sufficient mass for library construction. The library sequencing was performed on the Illumina novaseq 6000.