Effect of ?Np63 deletion on plasticity of post-injury lung basal cells

Basal cells were isolated from an influenza-infected non-tamoxifen treated Krt5CreERT2; RFP; ?Np63flox/flox mouse and cultured, allowing for inducible ?Np63 deletion in vitro. These basal cells were plated as monolayers, airway organoids, and alveolar organoids and treated with 1uM 4OHT for +4OHT (?Np63 KO) or equivalent volume DMSO for -4OHT (solvent only, ?Np63 WT). Technical triplicates of ?Np63 WT and ?Np63 KO were harvested for each growth condition for RNA sequencing. Overall design: Comparative transcriptomics of ±?Np63 post-injury basal cell monolayers, airway organoids, and alveolar organoids