The mRNA expression changes in neonatal primary mouse ventricular cardiomyocytes treated with epirubicin (2uM)

Epirubicin (EPI) is effective in the treatment of malignant cancers, but its application is limited by life-threatening cardiotoxicity. Iron homeostasis disturbance has been implicated in anthracycline-induced cardiotoxicity (AIC), and ferroptosis is involved in AIC which is dependent upon intracellular iron. However, the role and exact mechanisms of ferroptosis in the pathogenesis of epirubicin-induced cardiotoxicity (EIC) remain elusive. In this study, we aimed to investigate mechanisms underlying ferroptosis-driven EIC. Epirubicin triggered ferroptosis both in vivo and in cultured cardiomyocytes, and pretreatment with ferroptosis inhibitor, Ferrostatin-1(Fer-1) alleviates EIC. Microarray analysis was performed to screen for potential molecules involved in EIC in neonatal primary mouse ventricular cardiomyocytes (NMVMs). We found that the transcript level of ATP6V0A2, a subunit of vacuolar ATPase (V-ATPase), was significantly downregulated when NMVMs were subjected to EPI, which was v..., Total RNA was extracted from NMVMs treated with 2μM EPI for 24 hours and purified with an RNeasy mini kit (QIAGEN) according to the manufacturer's instructions. The microarray analysis was performed using Affymetrix GeneChip probe arrays (Thermo Fisher Scientific). P-values were adjusted with a false discovery rate of 5%. Controls were implemented at each step to ensure data quality., , # The mRNA expression changes in neonatal primary mouse ventricular cardiomyocytes treated with epirubicin (2uM)

[https://doi.org/10.5061/dryad.hdr7sqvs5](https://doi.org/10.5061/dryad.hdr7sqvs5)

## Description of the data and file structure

File name: **deg.anno.mRNA.xlsx**

Variables:

* ID: The indicated transcript ID of the mouse.
* logFC: log2FC, FC: fold change.
* Pvalue: The P-value is the probability of obtaining a result at least as extreme as the one that was observed, given that the null hypothesis is true. A p-value of 0.05 or lower is generally considered statistically significant.
* regulation: Whether the gene/transcript is up-regulated, down-regulated, or not regulated.
* up: The up-regulation genes in neonatal primary mouse ventricular cardiomyocytes (NMVMs) treated with epirubicin (2μM).
* down: The down-regulation genes in NMVMs treated with epirubicin (2μM).
* no: The down-regulation genes in NMVMs treated with epirubicin (2μM).
* ensembl: The indicated ID of the ...