Fos binding in hippocampal chromain 1 h after peritoneal KA administration

Fos induction is the most commonly used marker of neuronal activation. Here, we investigate the binding profile of Fos in the adult mouse hippocampus using antibodies specific against this transcription factor and the chromatin Immunoprecipitation coupled to DNA-sequencing (ChIP-seq) method. We generated profiles in basal conditions and 1 hour after neuronal stimulation. To trigger the syncronous activation of hippocampal exciatory neurons we treated the mice with kainic acid (KA) through peritoneal administration. This model of status epilepticus has been characterized in detail at the epigenomic level (Fernandez-Albert et al., 2019). Two-month-old C57BL/6J mice were treated with PBS (saline) of Kainic acid (KA, two consecutive intraperitoneal injections separated by 30 min at 10 mg/kg). One hour after the first kainate injection, mice with level 4 epileptic seizures were sacrificed with subsequent dissection of their hippocampi. A Dounce homogenizer was used to dissociate hippocampal tissue and the extract was incubated with anti-Fos antibody and used for ChIP-seq.