Age-related hearing los in Nox4 knockout mouse

The objective of the study was to explore the role of NOX4 in agen-related hearing loss. Hearing evaluation (with auditory brainstem responses, ABR) and cochlear gene expresión in Nox4 knockout mice compared to wild type mice, along age (2-15 months). ABR data were obtained from Nox4 knockout and wild type mice fom 2 to 15 month of age. Cochlear expression of Nox3, Nox4, Nrf2, Nlrp3, Il1b and Tnfa genes were determined by RT-qPCR in pooled simples (3 cochleae from 3 independent mice) in Nox4 knockout and wild type of 4 and 8 months of age.