Double knock-in pig models with elements of binary Tet-on and phiC31 intergrase systems for controllable and switchable gene expression

In summary, we have for the first time generated a Dox inducible and switchable tdTomato pig strain with dual knock-in at Hipp11 and Rosa26 locus. This pig model provide a versatile tool model to produce stable transgenic pigs with inducible overexpressing any gene of interest by using the technology of phiC31 integrase-mediated cassette exchange. Based on this approach, an inducible hKRASG12D-expressing pig model line was established and tumor formation was induced after Dox administration. We expect that the inducible tool pig model would greatly facilitate the production of controllable transgenic pigs, and broaden the applications of transgenic pigs in biomedicine and agriculture fields. Overall design: 18 samples for RNA-seq, including tumor tissues at nose, mouth, and scrotum from induced DIK pig (DIK-nose-1, 2, 3; DIK-mouth-1, 2, 3; DIK-scrotum-1, 2, 3) and normal nose, mouth, and scrotum tissues of wild-type pig (WT-nose-1, 2, 3; WT-mouth-1, 2, 3; WT-scrotum-1, 2, 3)