Role of activated PI3K-delta signaling in humoral immunity

We report the high-throughput profiling of murine naive B cells, germinal center (dark zone and light zone) B cells, and plasma cells transcriptome. By obtaining over 5 million bases of sequence, we generated genome-wide expression maps of cell subsets from WT mice and aPIK3CD mice. We find that activated PIK3CD signaling lead to significant alteration in gene expression of plasma cells. Transcriptome profiling of naïve B cells, germinal centers (dark zone and light zone) B cells, and plasma cells to determine the effect of activated PIK3CD signaling