Developing a novel quantitative parameter for characterizing spatial distribution of fish following exposure to chemicals and wastewater: behavioral Gini coefficient

To develop a quantiative assessment parameter for fish spatial distribution, we compared the spatial distribution parameters and general locomotor parameters of zebrafish after exposure to chemicals and wastewater. Based on the heatmap, we calculated the behavior Gini coefficient for quantitative assessment. The behavior Gini coefficient can more robustly identify alternations of spatial distribution of fish shoal and is an ideal parameter to quantitatively assess spatial distribution. Thus, the behavior Gini coefficient has promissing applications in toxicity tests for chemicals and wastewater and for automatic quanlity monitoring for surface water and aquaculture water.
The Supplementary Materials including the protocol, raw data and function run with MATLAB. Besides, the test data were also added for better use.