Nano-silica particles synergistically IgE-mediated mast cell activation

To deeply investigate the details of the nano-SiO2 effects, we examined the gene expression profile alterations after nano-SiO2 treatment in BMMCs. The difference analysis between the groups showed that 285 genes were significantly expressed after treatment with nano-SiO2. Compared with the blank group, both nano-SiO2 exposure and DNP-HSA stimulation increased the expression of genes related to the MAPK signaling pathway in mast cells to varying degrees. Overall design: Mature BMMCs were collected after treatment with SiO2 solution for transcriptome sequencing analysis. The experiment was divided into four groups: blank control,DNP-HSA, SiO2, and SiO2+DNP-HSA. We performed 2×150 bp paired-end sequencing (PE150) on an Illumina Novaseq™ 6000 (LC-BioTechnology CO., Ltd., Hangzhou, China) following the vendor's recommended protocol. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to analyze these differentially expressed genes. Bioinformatics analysis was performed using the OmicStudio tools at https://www.omicstudio.cn/tool.