Nafamostat mesylate protects against acute renal ischemia-reperfusion injury by alleviating mitochondrial dysfunction, inhibiting ferroptosis, and regulating proximal tubular cells: A multi-omics analysis

Twenty-one male rats were randomly divided into three groups: sham, I/R, and I/R+NM. Acute renal I/R injury models were created in the I/R and I/R+NM groups by right nephrectomy and clamping the left renal pedicle for 45 minutes. Rats in the I/R+NM group were intraperitoneally injected with 7.5 mg/kg of NM before modeling. Blood and kidney specimens were collected 24 hours after model establishment. RNA sequencing, KEGG and GO enrichment analyses, and single-cell sequencing were carried out to investigate the related mechanisms.