RNA-seq and ATAC-seq data of scaffold 3D bioprinted GBM. Transcriptional landscape of the interaction of human Mesenchymal Stem Cells with Glioblastoma in bioprinted co-cultures.

MSC can acquired characteristics of Cancer associated fibroblasts (CAF) by being cultured with conditioned medium from GBM cultures and thus are called MSCCAF. We co-cultured MSCCAF with patient derived GBM in a scaffold 3D bioprinted model. We studied the response to current GBM therapy (e.g. Temozolomide +/- radiation) on the co-cultures by bulk transcriptomic (RNA-sequencing) and epigenetic (ATAC-seq) analyses. RNA-seq Cells were harvested, cryopreserved and sent to Active Motif (https://www.activemotif.com) where RNA-seq were performed: RNA extraction, polyA+ RNA enrichment, directional library generation, QC of NGS library and next-generation sequencing using the Illumina platform. ATAC-seq Cells were harvested, cryopreserved and sent to Active Motif (https://www.activemotif.com/) where ATAC-seq were performed following the protocols from Buenrostro et al. 2013 Nat. Methods and Corces et al. 2017 Nat. Methods.