AlphaFold2 Multimer Structural Models for CtBP-Prospero Protein Interaction

Description This dataset contains AlphaFold2 multimer v3 structural predictions for the protein-protein interaction between C-terminal binding protein (CtBP) and Prospero transcription factor in Drosophila melanogaster. These models support the research published in "Metabolic control of enteroendocrine cell fate through a redox state sensor CtBP" by Rovenko et al. PMID: 40631244. Methods UniProt sequences were used as inputs for AlphaFold2 multimer structure prediction: sp|O46036|CTBP_DROME (full-length CtBP) sp|P29617|PROS_DROME (fragment EALSLVV, residues 1283-1289 of Prospero) Models were generated using the ColabFold framework implementing AlphaFold2 multimer v3 with standard protocols. The binding configuration used in the manuscript corresponds to the rank 1 model with high confidence metrics: average pLDDT of ~91, ipTM ~0.92, and actifpTM ~0.94. Dataset Contents This dataset includes all standard ColabFold outputs for reproducibility: 5 structural models (.pdb files) ranked by confidence Confidence score files (.json) for each model Multiple sequence alignment (.a3m file) Predicted aligned error data and visualizations (.json, .png) Coverage analysis and confidence plots (.png) Configuration and environment files Applications These structural models provide insights into the molecular basis of CtBP-Prospero interaction in enteroendocrine cell fate regulation and metabolic sensing mechanisms. Citation If you use these models, please cite: Rovenko et al. (PMID: 40631244), https://doi.org/10.1101/2025.06.30.662346