Differential Expression of miRNAs in Colorretal Cancer

This study aimed to investigate the miRNA's expression profile in order to identify possible biomarkers of colorretal cancer. We applied next generation sequencing to investigate the global expression profile of miRNAS from tissue samples of patients with cancer and external controls. 22 collected samples (7 patients' tissue, 7 patient's adjacent-tissue and 8 external controls) were prepared to be explored and passed a quality control pipeline (Trimmomatic software) and an alignment (STAR software) and read's count (HTSeq software) processes to perform differential expression (DESeq package), target genes (miRTarbase database), gene set enrichment (DAVID and Reactome databases) and miRNA-gene network analysis.