Summary of experimental validation results for the five designed sequencesa.

aThe sign of “+” and “−” indicates positive and negative experimental results respectively.bRMSD between the first I-TASSER model and the target scaffold.cProtein expression and solubility determined by visual identification via comassie stain gels.dPresence of secondary structural elements defined by circular dichroism.ePossession of a stable tertiary fold determined by the presence of secondary structural elements (CD) and NMR spectroscopy.fPercentage of α-helix residues decided by the CD spectra (the values in parentheses are the number in the scaffold structure.