RAG1:RAG2 recombinase binds immunoglobulin kappa locus in a BRWD1-dependent manner

Based on studies in mice, Bromodomain and WD repeat-containing protein 1 (BRWD1) is recruited to the Immunoglobulin kappa (IgK) locus through interaction with histone H3 acetylated at lysine residues K9 and K14 and phosphorylated at serine residue S10. ERK is implicated in histone H3 serine S10 phosphorylation at the IGK locus. BRWD1 enhances the recruitment of the RAG1:RAG2 recombinase complex to the IgK locus and positions it at recombination signal sequences within the IgK locus by proper positioning of the nucleosomes, in order for the V(D)J recombination to occur (Mandal et al. 2015). Please note that the listed amino acid residues in mature histone H3 match nascent histone H3 residues K10, K15 and S11, respectively. Amino acid positions in Reactome annotations of modified residues and Reactome systematic names correspond to positions in the nascent protein UniProt sequence.

基于对小鼠的研究，Bromodomain and WD repeat-containing protein 1（BRWD1）通过与其赖氨酸残基K9和K14发生乙酰化以及丝氨酸残基S10发生磷酸化的组蛋白H3相互作用，被招募至免疫球蛋白κ（IgK）位点。ERK在IGK位点组蛋白H3丝氨酸S10磷酸化过程中起到重要作用。BRWD1通过恰当定位核小体，增强了RAG1:RAG2重组酶复合物向IgK位点的招募，并将其定位于IgK位点的重组信号序列中，以便V(D)J重组得以发生（Mandal等，2015年）。请注意，成熟组蛋白H3中的氨基酸残基位置与新生组蛋白H3的K10、K15和S11位置相对应。Reactome对修饰残基的注释以及Reactome系统命名中的氨基酸位置与新生蛋白质UniProt序列中的位置相对应。