Extensive DNA methylation variation across a single ADPKD kidney

We have previously described global hypomethylation and site-specific DNA methylation changes which are associated with disease-state renal tissue in Autosomal Dominant Polycystic Kidney Disease (ADPKD). However, to-date there are no available data on the pattern of DNA methylation across an ADPKD kidney. Here we describe the methylome of eight individual cysts from a single ADPKD patient and peform an intra-individual analysis to identify regions of DNA methylation variability. Overall design: Here we generated single-nucleotide resolution methylomes of eight individual ADPKD cysts from a single patient using Reduced Representation Bisulfite Sequencing (RRBS).