Recognition of ubiquitinated histone H2A constitutes a treatment target for RSF1-driven ovarian cancer

RNA-seq results revealed that overexpression of RSF1, but not RSF1?UAB, induced a gene expression program enriched for cancer stem cell markers. Conversely, overexpression of a UAB domain decoy peptide reduced cell proliferation and blocked the cancer stem cell gene expression programs in OVCAR3 cells. Together, these studies reveal the critical role of recognizing H2Aub in RSF1-driven ovarian cancer and demonstrate that the interaction between RSF1 and H2Aub constitutes a druggable target for treating ovarian cancers with high levels of RSF1. Overall design: Expression profiling by high throughput sequencing