Effects of sf32 deletion in the biological activity of the virus.

a) Mean virus-induced mortalities following DNA injection. Values above the columns indicate means and those labeled with different letters are significantly different (p6 OBs of Sfbac, Sf32null and Sf32null-repair viruses. Values above columns indicate means and those labeled with different letters differed significantly (t-test, p8 OBs of Sfbac and Sf32null. Sf9 cells were serially infected (1∶5, 1∶25, 1∶125, and 1∶625) with ODVs released from OBs. ODV titers (ODV/ml) were calculated by end point dilution. Error bars indicate the standard error of the mean. d) Kaplan-Meier survival curves showing estimates of that the probability of an infected S. frugiperda larva surviving to different intervals following infection by each virus. Continuous and discontinuous lines represent Sfbac and Sf32null survival curves, respectively. e) OB production values in larvae infected with Sfbac and Sf32null viruses. Values above the columns indicate means. Error bars indicate the standard error of the mean. f) Dynamics of BV production through the time. Squares represent Sfbac values and triangles represent Sf32null values. Error bars indicate the standard deviation. No significant differences were observed in BV temporal production patterns between Sfbac and Sf32null viruses (p>0.05).