Michael Cammer, Phyllis Novikoff (2011) CIL:35282, Rattus rattus, hepatocyte. CIL. Dataset

Primary rat hepatocytes in culture fixed and stained for beta-tubulin. The microtubules form a network throughout the cytoplasm. In this image they are seen originating from a brighter and denser region adjacent to the cell nucleus.

This is a single optical section where the cell meets the substrate collected by widefield fluorescence microscopy with a Photometrics camera with a KAF1400 chip. The imaging was performed at a microscopy course at Woods Hole Oceanographic Institute in October 1993 and deconvolved on VayTec software running on a Macintosh computer. At the time, deconvolution was new and novel for use by biologists.

培养的初级大鼠肝细胞经固定和染色处理，用于检测微管蛋白。微管在细胞质内形成网络。在此图像中，微管起源于紧邻细胞核的一个更为明亮和致密的区域。这是一张单光切片图像，展示细胞与基底材料的接触，该图像是通过配备KAF1400芯片的Photometrics相机的宽场荧光显微镜收集的。成像工作于1993年10月在伍兹霍尔海洋学研究所的显微镜课程中完成，并在Macintosh计算机上运行的VayTec软件上进行了解卷积处理。当时，解卷积技术在生物学家中的应用尚属新颖且富有创新性。