Introducing Differential RNAseq mapping to track the phage infection process for Pseudomonas virus ɸKZ

Differential RNA-seq (dRNA-seq) was performed on Pseudomonas aeruginosa alone or shortly after iinfection with the jumbo phage phiKZ Pseudomonas aeruginosa PA01 was infected during the early exponential growth phase with phiKZ (MOI of 15). The infection was allowed to take place for 10min before cells were frozen in liquid nitrogen. RNA was extracted and after DNaseI treatment used for library construction according to the dRNA-seq protocol at Vertis Biotechnology (Germany).