Homo sapiens Transcriptome or Gene expression

Angelica polysaccharide (APS) is one of the major active components isolated from Angelica sinensis. Evidences suggested APS may have therapeutic potential for psoriasis. HaCaT cell line was commonly used as an in vitro model to study psoriasis. In the present study, RNA-sequencing (RNA-seq) was performed to investigate the underlying mechanism of APS. Different concentrations of APS (0mg/mL, 50 mg/L, 100 mg/L and 200mg/L) were incubated with HaCaT cells for 36 h and transcriptome sequenced. Comparison of the gene expression profiles between the CK group (i.e., Control group) and ASP groups revealed dramatic differences. All the differentially expressed genes (DEGs) were then classified into 20 expression profiles by trend analysis. Significant enriched trend clusters were then analyzed. Interestingly, cell proliferation related gene ontology (GO) terms were mostly dispersed in the profile 2 and 17. Based on our functional annotation results and reported literature, authors suspected that SERPINE1, SMAD6 and CTGF, together with TGF-ß, may closely relevant to the anti-proliferation effect of APS.