Figure S1 - 2-Hydroxyglutarate Production, but Not Dominant Negative Function, Is Conferred by Glioma-Derived NADP+-Dependent Isocitrate Dehydrogenase Mutations

Co-expression of IDH1-R132H with IDH1-WT does not lower NADP+-IDH activity more than vector alone. HOG cells were transfected with the indicated amounts of pCMV6 vectors to express IDH1-WT, IDH1-R132H, or vector alone (V), as indicated. 48 hours after transfection, cells were lysed and total cellular NADP+-IDH activity was determined at 1.3mM isocitrate. Results are from two independent measurements of lysates and are representative of two independent experiments. (PDF)