Single cell RNA sequence of liver tumors in hepatocyte-specific Kras mutant mice.

Tumor consisting cells in hepatocyte-specific Kras mutant mice were classified as tumor cells and stromal cells including macrophages, hepatic stellate cells, endothelial cells, and so on. Positive cells for STAT3-target genes, Il-6 family cytokines, and connective tissue growth factor were elucidated. Overall design: Two liver tumors in hepatocyte-specific Kras mutant mice (Alb-Cre +/- Kras G12D/+) were dissociated into a single-cell suspension and subjected to single cell RNA-sequence (scRNA-seq) to elucidate the mRNA expression profile of tumor-consisting cells.