Raw serum metabolomics from PCOS patients and healthy subjects

We collected serum samples from six PCOS patients and six healthy controls separately. The ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was carried out on PCOS patients and healthy controls to identify differential lipid metabolites. The UPLC-MS analysis mass spectrometry data were compared with the human metabolomics database (Human Metabolome Database). And the length of retention time was used to determine the structural annotation of metabolites. The XCalibur Quan Browser software was used to extract the peak area of ​​metabolites, and the peak area data was exported to Excel software for finishing. Raw serum metabolomics data includes lipid information collected in positive and negative ion modes.