Genome-wide mapping of H3K27me3 in Daxx-depleted cells

We discovered Daxx can negatively regulated H3K27me3 level via facilitating long non-coding RNA HOTAIR degradation. To further explore the biological consequence and search for affected genes, we used high throughput sequencing technology to demonstrate the enrichment of H3K27me3 on the human genome and target genes repression after depletion of Daxx. This study revealed a novel role of Daxx in regulating histone modifications via modulates long non-coding RNA stability. Overall design: Examination of H3K27me3 distribution in Daxx-depleted and untreated HEK293T cells