Transcriptomic signatures associated with underlying rapid changes in the early phase brain of bi-directional sex change in Trimma okinawae

Teleost fish exhibit remarkable sexual plasticity and divergent developmental systems, including hermaphroditism. One of the more fascinating models of sexual plasticity is socially controlled sex change, which is often observed in coral reef fish. The Okinawa rubble goby, Trimma okinawae, is a bi-directional sex-changing fish. It can rapidly change sex in either direction based on social circumstances. Although behavioral and neuroendocrine sex change occurs immediately and is believed to trigger gonadal changes, the underlying mechanisms remain poorly understood. In this study, we conducted a de novo transcriptome analysis of the T. okinawae brain and identified genes that are differentially expressed between the sexes and genes that were immediately controlled by social stimulation implicating sex change. Several genes showed concordant expression shifts regardless of the sex change direction and were associated with histone modification in nerve cells. These genes are known to funct..., , , # Transcriptome analysis of the Trimma okinawae brain

We conducted a de novo transcriptome analysis of the T. okinawae brain to detect the genes immediately controlled by social stimulation causing a sex change. Subsequently, protein-coding sequences were detected with TransDecoder using default parameters and remove the redundancy using CD-hit. We used RSEM with bowtie2 as a mapping program to calculate the mRNA abundance of the individual samples. Also, those contigs coding proteins were annotated by EggNoG2.

## Description of the data and file structure

Trinity_v265_L20T20S420A20M100.Trinity.fasta: The fasta file resulted from de-novo assembly using Trinity.
Trinity_v265_L20T20S420A20M100_onlyPepetide.fasta: The fasta file of putative protein-coding contigs filtered using TransDecoder and CD-hit.
EggNog_All_Anno.tsv: The annotation file of each protein coded in contigs using EggNOG2 mapper. The cells of \"-\" in this file represent no data.
RSEM.genes.results_Count_merge: The count ...