Integrated multi-omics approach revealed cellular senescence landscape

Cellular senescence is a complex multifactorial biological phenomenon that plays essential roles in aging, and aging-related diseases. During this process, the senescent cells undergo gene expression altering and chromatin structure remodeling. However, studies on the epigenetic landscape of senescence using integrated multi-omics approaches are limited. In this research, we performed ATAC-seq, RNA-seq, and ChIP-seq on different senescent types to reveal the landscape of senescence and identify the prime regulatory elements. Considering that human diploid 2BS fibroblasts are a widely used cell model to study cellular senescence and aging-related disorders, we employed growing 2BS cells and senescent 2BS cells as Replicative Cellular Senescence (RS) representatives to generate ATAC-seq libraries , which can sensitively measure high-resolution chromatin accessibility. For each age stage, we obtained two independent ATAC-seq replicates.